This video displays a 3D visualization of a dendrite from a striatopallidal neuron (iMSN) obtained through confocal microscopy using a Zeiss LSM 780 system and subsequent deconvolution. The fluorescent image stack reveals a dendrite with high resolution and clarity. The iMSNs displayed here were part of a study exploring the role of NMDA receptors in neuronal morphology and synaptic activity. The data revealed reduced dendritic arborization, lower spine density, and altered synaptic currents in NMDA-R-deficient neurons, shedding light on the mechanisms underlying synaptic plasticity and homeostatic regulation. The neuron shown in this video is from a wild-type (WT) mouse, serving as a control with normal NMDA receptor function. For more details, you can check the published study: J Neurosci. 2016 May 4;36(18):4976-92. Striatopallidal Neuron NMDA Receptors Control Synaptic Connectivity, Locomotor, and Goal-Directed Behaviors Laurie Lambot, Elena Chaves Rodriguez, Delphine Houtteman, Yuquing Li, Serge N Schiffmann, David Gall, Alban de Kerchove d’Exaerde. Techniques and Tools: 1) Confocal Microscopy: Serial optical sections (Z-stacks) captured with a Zeiss LSM 780 system, using multiple fluorophores to label dendrites and spines. 2) Image Deconvolution: Processed with Huygens-II software to enhance resolution and reduce noise. 3) 3D Reconstruction: Created with Imaris software to provide a comprehensive view of dendritic branches and spines. This visualization is an example of how neuroscience leverages imaging and computational tools to study neuronal networks and their structural features. #Neuroscience #ConfocalMicroscopy #3DVisualization #StriatopallidalNeurons #SynapticPlasticity #NMDAReceptors #DendriticArborization